Eugenia Pechkova

Evgeniya Peshkova (Eugenia Pechkova), born in Moscow, Russia, 24 May 1976, after taking her Doctoral degree in Chemistry at Moscow State Lomonosov University in 1998, enter the PhD course in Biophysics with the fellowship from Polo Nazionale Bioelettronica.
Research contract award for protein crystallization, project for young investigator of University of Genova.
After taking her PhD degree in Biophysics with the thesis entitled "Protein Crystallography By Thin Film Nanotechnology" at University of Genova in 2003, became firstly (2003-2006) Scientific Secretary of Fondazione EL.B.A. (Electronic Biotechnology Advanced) and then Scientific Director (2006-2008) and Principle Investigator of a big FIRB research grant on Organic Nanotecnology. In this context following a postdoctoral position she has acquired the scientific responsibility of the laboratory of Nanobiocrystallography at the Nanoworld Institute,University of Genoa.
In 2007 she worked as a Visiting Scientist at the European Synchrotron Radiation Facility (ESRF) in Grenoble both in Macromolecular crystallography group, Soft condensed matter and Nanofocusing group. Now is one of the PI of Radiation Damage BAG.
She participated as invited speaker to the international conferences on Crystallography, Nanobiotechnology and Medicine and carried out stages at Harvard University; IBM at Almaden, Jefferson Cancer Center, Univ of Massachusetts Medical School and UCLA.
From June 1, 2008 she has the permanent position of Assistant Professor of Biochemistry at the University of Genova Medical faculty.
Author of 37 international scientific publications (SCI), 2 patents; a textbook on "Proteomics and Nanocrystallography", chapters to books, co-author in "Biofisica e Propedeutica Biofisica" textbook "Biofisica" (Italian).

• Crystal XXII, Society of Crystallographers in Australia and New Zealand, Brisbane, Australia, July 2001
• VII International Conference on Advanced Material ICAM 2001 Cancun, Mexico, August 2001
• NANOBIONICS II From Molecules to Applications a Marburg University, Germania (Settembre 2002)
• ELBA/Max Planck Forums on Nanoscale Sciences and Technology, 2002, 2003, 2004
• Genomics, Proteomics and Bioinformatics for Medicine, Russain Academy of Sciences, St.Petersburg-Mosca, Russia (Giugno 2002)
• BNL Synchtroron, Symposia on Biologically Directed Self-Assembly of Nanomaterials: Synthesis and Characterization, Long Island, USA, May 2007
• CHESS Synchrotron Users Meeting, Cornell University, Itaca, USA, June 2008
• American Crystallographic Association International Congress, Knoxville, Tennessee, USA, June, 2008
• IASTED International conference on Nanotechnology and Applications (NANA 2008) September 2008, Crete, Greece, Chairman of the Panel session on Protein Nanocrystallography

• Pechkova E, Nicolini C, Brevetto Nazionale, 26.06.2001 "Metodo per la crescita accelerata di cristalli proteici su film sottili proteici"
• Pechkova E, Nicolini C, European Patent, 7.07.2002 "The new method of protein nucleation, crystallization and crystal growth acceleration based on the protein thin film template

• Invited Editor of the special issue of Journal of Synchrotron Radiation on Synchrotron radiation and Nanobiosciences, Volume 12, Novembre 2005, together with Claudio Nicolini
• Editor of third volume of Pan Stanford Publishing Series on Nanobiotechnology, entitled "Synchrotron Radiation and structural Proteomics", 2010, together with Christian Riekel

1. Pechkova E., Nicolini C., Accelerated protein crystal growth onto the protein thin film, Journal of Crystal Growth 231, 599-602, 2001.
2. Pechkova E., Nicolini C., Protein nucleation and crystallization by homologous protein thin film template, Journal of Cellular Biochemistry 85, 243-251, 2002.
3. Pechkova E., Nicolini C., From art to science in protein crystallization by means of thin film technology, Nanotechnology 13, 460-464, 2002.
4. Troitsky, V., Ghisellini P., Pechkova E., Nicolini C., DNASER II. Novel surface patterning for biomolecular microarray, IEEE Transaction on Nanobiosciences 1, 73-77, 2002.
5. Pechkova E., Zanotti G., Nicolini C., Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2, Acta Crystallographica D 59, 2133-2139. 2003.
6. Pechkova E., Nicolini C., Protein nanocrystallography: a new approach to structural proteomics, Trends in Biotechnology 22, 117-122, 2004.
7. Pechkova E., Nicolini C., Atomic structure of a CK2alpha human kinase by microfocus diffraction of extrasmall microcrystals grown with nanobiofilm template, Journal of Cellular Biochemistry 91, 1010-1020, 2004.
8. Nicolini C., Pechkova E., Nanocrystallography: an emerging technology for structural proteomics, Expert Review of Proteomics 1, 253-256, 2004.
9. Pechkova E., Tropiano G., Riekel C., Nicolini C., Radiation stability of protein crystals grown by nanostructured templates: synchrotron microfocus analysis, Spectrochimica Acta B 59, 1687-1693, 2004.
10. Pechkova E., Sivozhelezov V., Tropiano G., Fiordoro S., Nicolini C., Comparison of lysozyme structures derived from thin-film-based and classical crystals, Acta Crystallographica D61, 803-808, 2005.
11. Pechkova E., Fiordoro S., Fontani D., Nicolini C., Investigating crystal-growth mechanisms with and without LB template: protein transfer from LB to crystal, Acta Crystallographica D61, 809-812, 2005.
12. Pechkova E., Nicolini C., Synchrotron radiation and nanobiosciences - introductory overview, Journal of Synchrotron Radiation 12, 711, 2005.
13. Pechkova E., Roth S.V., Burghammer M., Fontani D., Riekel C., Nicolini C., microGISAXS and protein nanotemplate crystallization methods and instrumentation, Journal of Synchrotron Radiation 12, 713-716, 2005.
14. Pechkova E., Vasile F., Spera R., Fiordoro S., Nicolini C., Protein nanocrystallography growth mechanism and atomic structure of crystals induced by nanotemplates, Journal of Synchrotron Radiation 12, 772-778, 2005
15. Nicolini C., Pechkova E., Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: I microGISAXS and microdiffraction of P450scc, Journal of Cellular Biochemistry 97, 544-552, 2006.
16. Pechkova E., Nicolini C., Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: II microGISAX and microscopy of lysozyme, Journal of Cellular Biochemistry 97, 553-560, 2006.
17. Sivozhelezov V., Pechkova E., Nicolini C., Mapping electrostatic potential of a protein on its hydrophobic surface: implications for crystallization of cytochrome P450scc, Journal of Theoretical Biology 241, 73-80, 2006.
18. Nicolini C., Pechkova E., Nanostructured biofilms and biocrystals (a review), Journal of Nanoscience and Nanotechnology 6, 2209-2236, 2006.
19. Siodmiak J., Gadomski A., Pechkova E., Nicolini C., Computer model of a lysozyme crystal growth with/without nanotemplate - a comparison, International Journal of Modern Physics C 17, 1359-1366, 2006.
20. Pechkova E., Innocenzi P., Malfatti L., Kidchob T., Gaspa L.,Nicolini C., Thermal stability of lysozyme Langmuir-Schaefer films by FTIR spectroscopy, Langmuir 23, 1147-1151, 2007.
21. Pechkova E., Sivozhelezov V., Nicolini C., Protein thermal stability: the role of protein structure and aqueous environment, Archives of Biochemistry and Biophysics 466, 40-48, 2007.
22. Pechkova E., Sartore M., Giacomelli L., Nicolini C., Atomic force microscopy of protein films and crystals, Review of Scientific Instruments 78, 093704_1-093704-7, 2007.
23. Vasile F., Pechkova E., Nicolini C., Atomic structure of the beta-subunit of the translation initiation factor Aif2 from Archaebacteria sulfolobus solfataricus: high resolution NMR in solution, Proteins: Structure, Function and Bioinformatics 70, 1112-1115, 2008.
24. Sivozhelezov V., Braud C., Giacomelli L., Pechkova E., Giral M., Soulillou J.P., Brouard S., Nicolini C., Immunosuppressive drug-free operational immune tollerance in human kidney transplant recipient: II Non-statistical gene microarray analysis, Journal of Cellular Biochemistry 103, 1693-1706, 2008.
25. Braud C., Baeten D., Giral M., Pallier A., Ashton-Chess J., Braudeau C., Chevalier C., Lebars A., Lèger J., Moreau A., Pechkova E., Nicolini C., Soulillou J.P., Brouard S., Immunosuppressive drug-free operational immune tollerance in human kidney transplant recipient: I. Blood gene expression statistical analysis, Journal of Cellular Biochemistry 103, 1681-1692, 2008.
26. Pechkova E., Vasile F., Spera R., Nicolini C., Crystallization of alpha and beta subunits of IF2 translation initiation factor from Archabacteria Sulfolobus Solfataricus, Journal of Crystal Growth 310, 3767-3770, 2008.
27. Nicolini C., Bruzzese D., Sivozhelezov V., Pechkova E., Langmuir-Blodgett based lipase nanofilms of unique structure-function relationship, Biosystems 94, 228-232, 2008.
28. Pechkova E., Tripathi S., Spera R., Nicolini C., Groel crystal growth and characterization, Biosystems 94, 223-227, 2008.
29. Sivozhelezov V., Bruzzese D., Pastorino L., Pechkova E., Nicolini C., Increase of catalytic activity of lipase towards olive oil by Langmuir film immobilization of lipase, Enzyme and Microbial Technology 44, 72-76, 2009.
30. Pechkova E., Tripathi S., Nicolini C., microGISAXS of LB protein films: effect of temperature on long range order, Journal of Synchrotron Radiation 16, 330-335, 2009.
31. Pechkova E., Tripathi S., Ravelli R.G., McSweeney S., Nicolini C., Radiation stability of proteinase K grown by LB nanotemplate method, Journal of Structural Biology 168 , 409-418, 2009.
32. Nicolini C., Pechkova E., An overview of nanotechnology-based functional proteomics fro cancer and cell cycle progression, Anticancer Research 30, 2073-2080, 2010.
33. Pechkova E., Gebhardt R., Riekel C., Nicolini C., In situ GISAXS: I. Experimental setup for submicron study of protein nucleation and growth, Biophysical Journal 91, 1256-1261, 2010.
34. Gebhardt R., Pechkova E., Riekel C., Nicolini C., In situ microGISAXS: II. Thaumatin crystal growth kinetic, Biophysical Journal 91, 1262-1267, 2010.
35. Nicolini C., Pechkova E., Nanoproteomics for nanomedicine, Nanomedicine 5, 677-682, 2010.
36. Pechkova E., Nicolini C., Domain organization and properties of LB lysozyme crystals down to submicron size, Anticancer Research 30, 2745-2748, 2010.
37. Jovanovic V., Giacomelli L., Sivozhelezov V., Degauque N., Lair D., Soulillou J.P., Pechkova E., Nicolini C., Brouard S., AKT1 leader gene and downstream targets are involved in a rat model of kidney allograft tolerance, Journal of Cellular Biochemistry 111, 711-719, 2010.
38. Pechkova E., Nicolini C., In situ study of nanotemplate-induced grow th of lysozyme microcrystal by submicron GISAXS, Journal of Synchrotron Radiation 18, 287-292, 2011.
39. Racapé M, Bragazzi N, Sivozhelezov V, Danger R, Pechkova E, Duong Van Huyen JP, Soulillou JP, Brouard S, Nicolini C. SMILE silencing and PMA activation gene networks in HeLa cells: comparison with kidney transplantation gene networks. J Cell Biochem. 2012 Jun;113(6):1820-32.
40. Pechkova E, Scudieri D, Belmonte L, Nicolini C. Oxygen-bound Hell's gate globin I by classical versus LB nanotemplate method. J Cell Biochem. 2012 Jul;113(7):2543-8.
41. Bragazzi NL, Pechkova E, Scudieri D, Terencio TB, Adami M, Nicolini C. Recombinant laccase: II. Medical biosensor. Crit Rev Eukaryot Gene Expr. 2012;22(3):197-203.
42. Belmonte L, Pechkova E, Tripathi S, Scudieri D, Nicolini C. Langmuir-Blodgett nanotemplate and radiation resistance in protein crystals: state of the art. Crit Rev Eukaryot Gene Expr. 2012;22(3):219-32.
43. Nicolini C, Bragazzi N, Pechkova E. Nanoproteomics enabling personalized nanomedicine. Adv Drug Deliv Rev. 2012 Oct;64(13):1522-31.
44. Pechkova E, Sivozhelezov V, Belmonte L, Nicolini C. Unique water distribution of Langmuir-Blodgett versus classical crystals. J Struct Biol. 2012 Oct;180(1):57-64.
45. Nicolini C, Bezerra T, Pechkova E. Protein nanotechnology for the new design and development of biocrystals and biosensors. Nanomedicine (Lond). 2012 Aug;7(8):1117-20.
46. Nicolini C, Adami M, Sartore M, Bragazzi NL, Bavastrello V, Spera R, Pechkova E. Prototypes of newly conceived inorganic and biological sensors for health and environmental applications. Sensors (Basel). 2012 Dec 12;12(12):17112-27.
47. Nicolini C, Bruzzese D, Cambria MT, Bragazzi NL, Pechkova E. Recombinant laccase: I. Enzyme cloning and characterization. J Cell Biochem. 2013 Mar;114(3):599-605.
48. Nicolini C, Correia TB, Stura E, Larosa C, Spera R, Pechkova E. Atomic force microscopy and anodic porous allumina of nucleic acid programmable protein arrays. Recent Pat Biotechnol. 2013 Aug;7(2):112-21.
49. Pechkova E, Bragazzi N, Bozdaganyan M, Belmonte L, Nicolini C. A review of the strategies for obtaining high quality crystals utilizing nanotechnologies and space. Crit Rev Eukaryot Gene Expr. 2014;24(4), in press, DOI: 10.1615/CritRevEukaryotGeneExpr.2014008275.
50. Bozdaganyan M, Bragazzi N, Pechkova E, Shaitan K, Nicolini C. Identification Of Best Protein Crystallization Methods By Molecular Dynamics. Crit Rev Eukaryot Gene Expr. 2014;24(4), in press, DOI: 10.1615/CritRevEukaryotGeneExpr.2014010201.
51. Nicolini C, Bragazzi NL, Pechkova E. From Nanobiotechnology to Organic and Biological Monitoring of Health and Environment for Biosafety. Biosafety. 2013;2:116.
52. Spera R, Vasile F, Pechkova E, Nicolini C. Correlation of Changes of Cho–K1 Cells Metabolism to Changes in Protein Expression in Camp Differentiation. Altern Integ Med. 2013;2:105.
53. Nicolini C, Spera R, Festa F, Belmonte L, Chong S, LaBaer J, Pechkova E. Mass Spectrometry and Florescence Analysis of Snap-Nappa Arrays Expressed Using E. coli Cell_Free Expression System. J Nanomed Nanotechnol. 2013;4:181.
54. Pechkova E, Belmonte L, Riekel C, Popov D, Koenig C, Nicolini C. Laser-Microdissection of Protein Crystals Down to Submicron Dimensions. J Nanomed Nanotechol. 2013;S15:002.
55. Nicolini C, Bragazzi N, Pechkova E. From Nanobiotechnology to Organic and Biological Monitoring of Health and Environment for Biosafety. J Bioanal Biomed. 2013;5:108-117.
56. Nicolini C, Belmonte L, Maksimov G, Brazhe N, Pechkova E. In situ Monitoring By Raman Spectroscopy of Lysozyme Conformation during “Nanotemplate” Induced Crystallization. J Microb Biochem Technol. 2013;6:009-016.
57. Nicolini C, Bragazzi NL, Pechkova E, Lazzari R. Ab Initio Semi- Quantitative Analysis of Micro-Beam Grazing-Incidence Small-Angle X-Ray Scattering (M-GISAXS) during Protein Crystal Nucleation and Growth. J Proteomics Bioinform. 2014;7:064-070.
58. Spera R, Festa F, Bragazzi NL, Pechkova E, LaBaer J, Nicolini C. Conductometric monitoring of protein-protein interactions. J Proteome Res. 2013 Dec 6;12(12):5535-47.
59. Bragazzi NL, Spera R, Pechkova E, Nicolini C. NAPPA-Based Nanobiosensors for the Detection of Proteins and of Protein-Protein Interactions Relevant to Cancer. J Carcinog & Mutagen. 2014;5:166.
60. Nicolini C, Belmonte L, Riekel C, Koenig C, Pechkova E. Langmuir-blodgett nanotemplate crystallization combined to laser-microfragmentation uniquely characterize proteins crystals by synchrotron microdiffraction. Am. J. Biochem. Biotechnol. 2014;10:22-30.
61. Bragazzi NL, Pechkova E, Nicolini C. Proteomics and proteogenomics approaches for oral diseases. Adv Protein Chem Struct Biol. 2014;95:125-62.
62. Pechkova E, Bragazzi NL, Nicolini C. Advances in nanocrystallography as a proteomic tool. Adv Protein Chem Struct Biol. 2014;95:163-91.
63. Nicolini C, Pechkova E, New Trends in Protein Nanocystallography by Cell Free Expression, APA Microarras, LB Nanotemplate and Montecarlo Simulation: Review, Journal Micro and Biochemical Technology, 2014;6(7), 366-369.

1. Pechkova E., Nicolini C., Proteomics and Nanocrystallography, Kluwer Academic Plenum Publishers, 1-190, 2003.
2. Pechkova E., Nicolini C., From Art to Science in Protein crystallography by means of nanotechnology - one year later, invited chapter, in Trends In Nanotechnology Research, Nova Science Publishers, 31-50, 2004.
3. Pechkova E., Nicolini C., Synchrotron Radiation and Nanobiosciences, Special Issue Journal of Synchrotron Radiation 12, 2005.
4. Gadomski A., Santamaria-Holek I., Kruszewska N., Uher J.J., Pawlak Z., Oloyede A., Pechkova E., Nicolini C., Can modern statistical mechanics unravel so me practical problems encountered in model biomatter aggregation emerging in internal- and external-friction conditions?, In Statistical Mechanics R esearch (Ed. Byung-Soo Kim), ISBN 978-2-60456-029-9, Nova Science Publishers Inc., New York (2008) pp. 44-98.
5. Pechkova, E., Capitolo 3 in Biofisica e Propedeutica Biofisica, C. Nicolini, Aracne Editrice, 2009 (ISBN 978-88-548-2373-0).
6. Pechkova E., Chong S., Tripathi S., Nicolini C., Cell free expression and APA for NAPPA and protein crystallography, in Functional Proteomics and Nanotechnology-based Microarrays, (Eds. Nicolini C., LaBaer J.) Pan Stanford Series on Nanobiotechnology, London - New York - Singapore, Volume 2, Chapter 7, pp. 121-147, 2010.
7. Pechkova, E., Riekel, C., Structural Proteomics and Synchrotron Radiation, Pan Stanford Series on Nanobiotechnology Volume 3, (Singapore, New York and London) in press 2011.
8. Nicolini C., Riekel C., Pechkova E. Growth and organization of Langmuir-Blodgett protein crystals via in situ GISAXS, laser-microdissection, nanodiffraction, Raman spectroscopy and atomic force microscopy. In: Synchrotron Radiation and Structural Proteomics, (Eds E. Pechkova, C. Riekel), Volume 3 Pan Stanford Series on Nanobiotechnology (Singapore), in press 2011.
9. Vasile F., Pechkova E., Nicolini C. Structural analysis of the beta-subunit of the translation initiation factor AIF2 from different species: role of Zn ions. In: Synchrotron Radiation and Structural Proteomics, (Eds E. Pechkova, C. Riekel), Volume 3, Pan Stanford Series on Nanobiotechnology (Singapore), in press 2011.
10. Pechkova E., McSweeney S., Nicolini C. Atomic structure and radiation resistance of Langmuir-Blodgett protein crystals. In: Synchrotron Radiation and Structural Proteomics, (Eds E. Pechkova, C. Riekel), Volume 3, Pan Stanford Series on Nanobiotechnology (Singapore), pp. 249-275, 2011.
11. Sivozhelezov V., Pechkova E., Nicolini C. Molecular modeling to facilitate protein crystallization. In: Synchrotron Radiation and Structural Proteomics, (Eds E. Pechkova, C. Riekel), Volume 3, Pan Stanford Series on Nanobiotechnology (Singapore), in press 2011.
12. Nicolini C., Pechkova E. Aspetti Biochimici su Struttura e Funzione delle Biomolecole. In: Biochimica Umana Con Schede Cliniche. Editore: Francesco Salvatore. Capitolo 24, p. 775-798, Napoli: Idelson Gnocchi Edizioni Scientifiche.